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KMID : 0384119910110030675
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Korean Journal of Clinical Pathology
1991 Volume.11 No. 3 p.675 ~ p.684
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Characterzation of Autoantibodies in the Sera of the SLE Patients with Positive Anti-DNA Antibody Test
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H. S. Kim
M. K. Lee/S. Y. Lee
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Abstract
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There are growing interests about autoimmune diseases including systemic lupus erythematosus(SLE) and great efforts have been made to develop sensitive and specific diagnostic tests for the autoimmune diseases. To date, autoantibody detection method such as indirect immunofluorescent antinuclear antibody(ANA) test has been widely used for the diagnosis of the autoimmune diseases. However this method gives us only the information on the pattern and titer of ANA present. In this study, attempts were made to analyze and characterize the components of autoantibody specificities in 20 patients with SLE who showed anti-DNA positivity by western blot analysis using rabbit thymus extract(RTE) and bovine spleen extract (BSE) as antigens. The results are as follows. 1. Immunoblot analysis of SLE patients showed 2-8 discrete immunoreactive protein bands in all of the patients tested, whereas only one band was detected in one of the 10 normal control subjects. 2. The frequent antibody bands in SLE patients were 52 kDa, 2 kDa, 26 kDa with RTE antigen, and 95 kDa, 64 kDa, 47kDa, 22kDa with BSE antigen. 3. Patients having high ANA titers showed tendency of increased number and intensity of bands in immunoblot. Similar picture was noted with anti-DNA antibody. Taken all the results together, we confirmed that there are up to 8 different kinds of antibody specificities in SLE patients. Further study is needed for evaluation of the clinical significance of these antibodies in SLE patients as well as comparison with other autoimmune diseases.
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